Production of Lignocellulolytic Enzymes and Biomass of Trametes versicolor from Agro-Industrial Residues in a Novel Fixed-Bed Bioreactor with Natural Convection and Forced Aeration at Pilot Scale

Solid-state fermentation requires the development of more efficient cultivation systems for its industrial application. The objective this work was to evaluate effect aeration regime on production biomass and several lignocellulolytic enzymes (laccase, manganese peroxidase, endoxylanase, β-glucosidase, total cellulases) by Trametes versicolor in a novel fixed-bed solid-state pilot-scale bioreactor. Three regimes were assessed: natural convection, low level (10 min every 6 h at 10 L/h air flowrate), high (1 flowrate). mushroom grown medium based lignocellulosic residues. design bioreactor, as well control aeration, humidity, temperature beds, allowed T. grow properly medium, reaching maximum 204.7 mg/g dry solid (ds). influence that had determined. Low forced favored obtaining highest titers laccase (6.37 U/g ds) compared convection level. greatest lignin degradation also verified regime. For first time, pilot scale reported